Skin & Eye Safety Testing

Topical Care Range

Clinical report 1 a. – Skin safety studies

​Type of study: Controlled occlusive patch test
Date: 14-09-2023​
Subjects: n = 54 ​
Age: 18-74 yr​
Gender: male & female (89% females)​
Subject groups: participants with self-reported sensitive (56%) or normal (44%) skin, and history of allergies (28%)​
Duration: 24 hr ​
Dose: 10%
Treatment: LE Care Products (right arm)​
Control: distilled water (left arm) ​
Assessments: dermatological scores for redness, thickening, bumps, or broken skin, rating scale (0-4)​

Outcomes: ​No visual signs of any irritation to any product in any subject compared to controls, with 0.0 score for all subjects for all tested products

https://littleetoile-sg.com/wp-content/uploads/2022/12/Skin-irritation-scores-after-24hr-exposure-of-arm-skin-to-LEC-products-01.png

Clinical report 2 a. – Skin safety studies

​Type of study: Controlled occlusive patch test ​
Date: 09-10-2023​
Subjects: n = 54​
Age: 44-77 yr​
Gender: Male & female (80% females)​
Subject groups: Participants with self-reported sensitive skin (81%) or normal skin (19%); participants with history of allergy (35%) or none (65%)​
Duration: 24 hr ​
Dose: 10%
Treatment: LE Care (right arm)​
Control: Distilled water (left arm) ​
Assessments: Dermatological scores for redness, thickening, bumps, or broken skin, using a rating scale (0-4)​

Outcomes: ​There were no visual signs of any irritation to any product in any subject compared to controls, with 0.0 score for all subjects for all tested products

https://littleetoile-sg.com/wp-content/uploads/2024/03/Skin-irritation-scores-after-24hr-exposure-of-arm-skin-to-LEC-products-02.png

Scientific report 1 b. – Eye safety studies

​Type of study: Ocular irritation test (OECD No. 496)​
Duration: 24 hrs​
Dose: up to 5% ​
Treatment: LE Care products​
Assessments: small amounts of sample were applied to a semi-permeable membrane that mimics the cornea. Any damage to the membrane after 24hrs is detected using digital imaging and given a score of 0-80. Acceptable levels are <12 units.

https://littleetoile-sg.com/wp-content/uploads/2022/12/Ocular-Irritancy-Score-after-24-Hour_Ocular-Irritancy-Score-24-hours-Exposure-to-LEC-products.png

Scientific report 1 b. – Eye safety studies

​Type of study: Ocular irritation test (OECD No. 496)​
Duration: 24 hrs​
Dose: up to 5% ​
Treatment: LE Care products​
Assessments: small amounts of sample were applied to a semi-permeable membrane that mimics the cornea. Any damage to the membrane after 24hrs is detected using digital imaging and given a score of 0-80. Acceptable levels are <12 units.

https://littleetoile-sg.com/wp-content/uploads/2022/12/Ocular-Irritancy-Score-after-24-Hour-Exposure-to-LEC-products-01.png

Technical report 3a. – dechlorination assay

​Type of study: Laboratory-based Color Change Assay
Assessments: ​Swimprotect 2-in-1 was tested with standardized method using increasing levels of sodium hypochlorite solution (up to 5 ppm) spanning levels found in chlorinated pools. ​The product was mixed with the chlorinated water, and color change reactions (indicating changes in chlorine) was measured using a digital meter.​

Outcomes: SWIMPROTECT 2-in-1 shows a repeatable effect on neutralizing dissolved chlorine, up to levels that exceed chlorinated pool water.

https://littleetoile-sg.com/wp-content/uploads/2022/12/Ocular-Irritancy-Score-after-24-Hour_Reduction-in-Dissolved-Chlorine-with-Icreasing-Level-of-swimprotect-2in1-1.png